Okada Y, Koizumi K, Kitahata S
Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Japan.
Carbohydr Res. 1994 Feb 17;254:1-13. doi: 10.1016/0008-6215(94)84238-8.
Trace amounts of trimaltosyl-cyclomaltoheptaoses (trimaltosyl-beta-cyclodextrins, trimaltosyl-beta CDs) were found in a mixture of maltosyl-cyclomaltoheptaoses (maltosyl-beta-cyclodextrins, maltosyl-beta CDs) prepared from maltose and cyclomaltoheptaose (beta-cyclodextrin, beta CD) through the reverse action of Klebsiella pneumoniae pullulanase. Five positional isomers of trimaltosyl-beta CD were isolated by high-performance liquid chromatography (HPLC) on a reversed-phase column and a graphitized carbon column. For the structural analysis of 6(1), 6(2), 6(3)-, 6(2), 6(5)-, and 6(1), 6(3), 6(5)-tri-O-maltosyl-beta CDs, an enzymic method using glucoamylolysis, followed by hydrolysis with Bacillus subtilis saccharifying alpha-amylase, was applied. Although 6(1), 6(2), 6(4)- and 6(1), 6(2), 6(6)-substituted isomers were indistinguishable by this method, these isomers were distinguished clearly by digestion of branched oligosaccharides produced from each isomer by the aforesaid method, with B. stearothermophilus neopullulanase or with glucoamylase. The resulting hydrolysates were analyzed by HPLC on an amino derivatized column and by fast-atom bombardment spectrometry (FABMS). The chromatographic behavior and spectral data (13C NMR and FABMS) of five positional isomers of trimaltosyl-beta CD are described.
在通过肺炎克雷伯氏菌支链淀粉酶的逆反应由麦芽糖和环麦芽七糖（β-环糊精，β-CD）制备的麦芽低聚环麦芽七糖（麦芽低聚-β-环糊精，麦芽低聚-β-CD）混合物中发现了痕量的麦芽三糖基环麦芽七糖（麦芽三糖基-β-环糊精，麦芽三糖基-β-CD）。通过反相柱和石墨化碳柱上的高效液相色谱（HPLC）分离出麦芽三糖基-β-CD的五种位置异构体。对于6(1), 6(2), 6(3)-、6(2), 6(5)-和6(1), 6(3), 6(5)-三-O-麦芽三糖基-β-CD的结构分析，采用了葡糖淀粉酶解的酶法，随后用枯草芽孢杆菌糖化α-淀粉酶水解。尽管通过该方法无法区分6(1), 6(2), 6(4)-和6(1), 6(2), 6(6)-取代的异构体，但通过用嗜热脂肪芽孢杆菌新支链淀粉酶或葡糖淀粉酶消化由上述方法从每种异构体产生的支链寡糖，可以清楚地区分这些异构体。通过氨基衍生化柱上的HPLC和快原子轰击质谱（FABMS）对所得水解产物进行分析。描述了麦芽三糖基-β-CD的五种位置异构体的色谱行为和光谱数据（13C NMR和FABMS）。
