咖啡豆中α-半乳糖苷酶催化转半乳糖基化反应产生的新型异质支链环麦芽寡糖（环糊精）的分离与表征
Isolation and characterization of novel heterogeneous branched cyclomalto-oligosaccharides (cyclodextrins) produced by transgalactosylation with alpha-galactosidase from coffee bean.
作者信息
Koizumi K, Tanimoto T, Okada Y, Hara K, Fujita K, Hashimoto H, Kitahata S
机构信息
Faculty of Pharmaceutical Sciences, Mukogawa Women's University, Nishinomiya, Japan.
出版信息
Carbohydr Res. 1995 Nov 30;278(1):129-42. doi: 10.1016/0008-6215(95)00240-0.
Transgalactosylated derivatives of cyclomalto-hexaose (alpha CD), -heptaose (beta CD), and -octaose (gamma CD) were synthesized by alpha-galactosidase from coffee bean using melibiose as a donor and alpha CD, beta CD or gamma CD as an acceptor. Mono- and di-O-alpha-D- galactosylated CDs were isolated and purified by HPLC. Their structures were elucidated by fast-atom bombardment mass spectrometry (FABMS) and 13C NMR spectroscopy. For structural determination of positional isomers of 6(1),6n-di-O-alpha-D-galactosyl-CDs, digestion products with cyclodextrin glucanotransferase were analyzed by HPLC and FABMS.
以蜜二糖为供体，α-环糊精（α-CD）、β-环糊精（β-CD）或γ-环糊精（γ-CD）为受体，利用咖啡豆中的α-半乳糖苷酶合成了环麦芽六糖（α-CD）、环麦芽七糖（β-CD）和环麦芽八糖（γ-CD）的转半乳糖基化衍生物。通过高效液相色谱法（HPLC）分离并纯化了单-O-α-D-半乳糖基化环糊精和二-O-α-D-半乳糖基化环糊精。通过快原子轰击质谱法（FABMS）和13C核磁共振光谱法阐明了它们的结构。对于6(1),6n-二-O-α-D-半乳糖基化环糊精位置异构体的结构测定，通过HPLC和FABMS分析了环糊精葡糖基转移酶的消化产物。
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