Hinton D P, Johnson C S
Department of Chemistry, University of North Carolina, Chapel Hill 27599-3290.
Chem Phys Lipids. 1994 Feb;69(2):175-8. doi: 10.1016/0009-3084(94)90039-6.
It is demonstrated that the fraction of sucrose entrapped in the aqueous compartment of phospholipid vesicles and the tracer diffusion coefficient of the vesicles can be determined simultaneously with diffusion ordered 2D NMR spectroscopy (DOSY). The sizes of the vesicles are related to the diffusion coefficients by the Stokes-Einstein equation. This method displays 1H-NMR peaks for sucrose in the diffusion dimension at positions corresponding to free and entrapped sucrose, and the areas of these peaks are proportional to the amount of free and entrapped sucrose, respectively. The results were verified by a 1D NMR experiment in which the sucrose peaks were integrated before and after dialysis to remove the external sucrose.
结果表明，利用扩散排序二维核磁共振波谱法（DOSY）可同时测定包封于磷脂囊泡水相中的蔗糖比例以及囊泡的示踪扩散系数。囊泡大小通过斯托克斯-爱因斯坦方程与扩散系数相关联。该方法在扩散维度上对应游离蔗糖和包封蔗糖的位置显示出蔗糖的1H-NMR峰，且这些峰的面积分别与游离蔗糖和包封蔗糖的量成正比。通过一维NMR实验对结果进行了验证，在该实验中，透析前后对蔗糖峰进行积分以去除外部蔗糖。
