Cao Ruge, Nonaka Airi, Komura Fusae, Matsui Toshiro
Division of Bioresources and Biosciences, Faculty of Agriculture, Graduate School of Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan.
Division of Bioresources and Biosciences, Faculty of Agriculture, Graduate School of Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan.
Food Chem. 2015 Mar 15;171:8-12. doi: 10.1016/j.foodchem.2014.08.105. Epub 2014 Sep 4.
This work focuses on a quantitative analysis of sucrose using diffusion ordered-quantitative (1)H-nuclear magnetic resonance spectroscopy (DOSY-qNMR), where an analyte can be isolated from interference based on its characteristic diffusion coefficient (D) in gradient magnetic fields. The D value of sucrose in deuterium oxide at 30°C was 4.9 × 10(-10)m(2)/s at field gradient pulse from 5.0 × 10(-2) to 3.0 × 10(-1)T/m, separated from other carbohydrates (glucose and fructose). Good linearity (r(2)=0.9999) was obtained between sucrose (0.5-20.0 g/L) and the resonance area of target glucopyranosyl-α-C1 proton normalised to that of cellobiose C1 proton (100.0 g/L, as an internal standard) in 1D sliced DOSY spectrum. The DOSY-qNMR method was successfully applied to quantify sucrose in orange juice (36.1 ± 0.5 g/L), pineapple juice (53.5 ± 1.1g/L) and a sports drink (24.7 ± 0.6g/L), in good agreement with the results obtained by an F-kit method.
这项工作聚焦于使用扩散排序定量（1）H核磁共振波谱法（DOSY-qNMR）对蔗糖进行定量分析，在该方法中，分析物可基于其在梯度磁场中的特征扩散系数（D）与干扰物分离。在30°C的重水中，当场梯度脉冲从5.0×10（-2）到3.0×10（-1）T/m时，蔗糖的D值为4.9×10（-10）m（2）/s，可与其他碳水化合物（葡萄糖和果糖）区分开来。在1D切片DOSY谱中，蔗糖（0.5 - 20.0 g/L）与目标吡喃葡萄糖基-α-C1质子的共振面积（相对于纤维二糖C1质子的共振面积（100.0 g/L，作为内标）进行归一化）之间获得了良好的线性关系（r（2） = 0.9999）。DOSY-qNMR方法成功应用于定量橙汁（36.1 ± 0.5 g/L）、菠萝汁（53.5 ± 1.1 g/L）和运动饮料（24.7 ± 0.6 g/L）中的蔗糖，结果与F试剂盒法所得结果高度一致。
