Takai K, Horie N, Yamaizumi Z, Nishimura S, Miyazawa T, Yokoyama S
Department of Biophysics and Biochemistry, Graduate School of Science, University of Tokyo, Japan.
FEBS Lett. 1994 May 9;344(1):31-4. doi: 10.1016/0014-5793(94)00354-8.
Codon recognition by Escherichia coli tRNA(Leu)4 and tRNA(Leu)5 was investigated by analysis of the competition between two aminoacyl-tRNA species in an in vitro protein synthesis. Both tRNA species strictly obey the wobble rule when they are in competition with other tRNA species. This is probably due to the post-transcriptional modifications at the first position of the anticodon of these tRNA(Leu) species, supporting the proposal that the conformational rigidity of post-transcriptionally modified pyrimidine nucleotides guarantees the correct codon recognition.
通过分析体外蛋白质合成中两种氨酰-tRNA 物种之间的竞争，研究了大肠杆菌 tRNA(Leu)4 和 tRNA(Leu)5 对密码子的识别。当这两种 tRNA 物种与其他 tRNA 物种竞争时，它们都严格遵守摆动规则。这可能是由于这些 tRNA(Leu) 物种反密码子第一位的转录后修饰，支持了转录后修饰的嘧啶核苷酸的构象刚性保证正确密码子识别的提议。
