Takai K, Okumura S, Hosono K, Yokoyama S, Takaku H
Department of Industrial Chemistry, Chiba Institute of Technology, Narashino, Japan.
FEBS Lett. 1999 Mar 19;447(1):1-4. doi: 10.1016/s0014-5793(99)00255-0.
5-Methoxyuridine was introduced into the first position of the anticodon of the unmodified form of tRNA(1Ser) from Escherichia coli. The codon reading efficiencies of this tRNA (tRNA(5-methoxyuridine UGA)) relative to those of the unmodified counterpart (tRNA(UGA)) were measured in a cell-free translation system. tRNA(5-methoxyuridine UGA) was more efficient than tRNA(UGA) in the reading of the UCU and UCG codons and was less efficient in the reading of the UCA codon. Thus, the single modification of U to 5-methoxyuridine can enhance the wobble readings.
5-甲氧基尿苷被引入到来自大肠杆菌的未修饰形式的tRNA(1Ser)反密码子的第一位。在无细胞翻译系统中测量了这种tRNA（tRNA(5-甲氧基尿苷UGA)）相对于未修饰对应物（tRNA(UGA)）的密码子阅读效率。tRNA(5-甲氧基尿苷UGA)在阅读UCU和UCG密码子方面比tRNA(UGA)更有效，而在阅读UCA密码子方面效率较低。因此，将尿苷单修饰为5-甲氧基尿苷可以增强摆动阅读。
