Pautova L V, Yakubov L A, Karamyshev V N, Vlassov V V
Institute of Bioorganic Chemistry, Siberian Division of Russian Academy of Sciences, Novosibirsk.
FEBS Lett. 1994 May 9;344(1):96-8. doi: 10.1016/0014-5793(94)00360-2.
The interaction of reactive derivatives of oligonucleotides bearing a 4-[(N-2-chloroethyl-N-methyl)amino]benzylamin residue at the 5'-terminal phosphate with serum blood proteins has been investigated. It was found that the compounds react with serum albumin and immunoglobulins M and G, the reactivity increasing in the order: albumin < IgG < IgM. The reactions with immunoglobulins were inhibited in the presence of different oligonucleotides, DNA and heparin, suggesting the oligonucleotide binding to some cationic region of the proteins. Myoglobin inhibited the interaction of oligonucleotide derivatives with myoglobin-specific monoclonal antibodies which indicates that the derivatives interact with the proteins within or near the antigen binding site.
对在5'-末端磷酸处带有4-[(N-2-氯乙基-N-甲基)氨基]苄基氨基残基的寡核苷酸反应性衍生物与血清血蛋白的相互作用进行了研究。发现这些化合物与血清白蛋白以及免疫球蛋白M和G发生反应，反应活性按以下顺序增加：白蛋白＜免疫球蛋白G＜免疫球蛋白M。在不同的寡核苷酸、DNA和肝素存在下，与免疫球蛋白的反应受到抑制，这表明寡核苷酸与蛋白质的某些阳离子区域结合。肌红蛋白抑制了寡核苷酸衍生物与肌红蛋白特异性单克隆抗体的相互作用，这表明衍生物与抗原结合位点内或其附近的蛋白质发生相互作用。
