López-Amorós R, Comas J, Carulla C, Vives-Rego J
Departament de Microbiologia, Universitat de Barcelona, Spain.
FEMS Microbiol Lett. 1994 Apr 1;117(2):225-9. doi: 10.1111/j.1574-6968.1994.tb06769.x.
Flow cytometry was used to study the lag, exponential, stationary and death phases of non-fixed cultures of Escherichia coli. Fluctuations in the forward angle scatter signal (FALS) were compared with cell size as measured by scanning electron microscopy at low temperature and image analysis. A correlation between FALS and cell size was not observed, although a correlation (r = -0.8) was obtained between FALS and the age of the culture for the first eleven days of incubation. Marked increases in FALS were observed during the lag phase, which were attributed both to changes in size and changes in structure or chemical composition. The distribution of FALS for all culture phases was asymmetric, and was associated with the cell size distribution.
流式细胞术用于研究大肠杆菌非固定培养物的迟缓期、对数期、稳定期和死亡期。将前向角散射信号（FALS）的波动与通过低温扫描电子显微镜和图像分析测量的细胞大小进行比较。尽管在培养的前11天FALS与培养物的年龄之间获得了相关性（r = -0.8），但未观察到FALS与细胞大小之间的相关性。在迟缓期观察到FALS显著增加，这归因于大小变化以及结构或化学成分的变化。所有培养阶段的FALS分布不对称，并且与细胞大小分布相关。
