Clausen M, Lamb C J, Megnet R, Doerner P W
Plant Biology Laboratory, Salk Institute, San Diego, CA 92186-5800.
Gene. 1994 May 3;142(1):107-12. doi: 10.1016/0378-1119(94)90363-8.
The yeast enzyme phenylacrylic acid decarboxylase (PAD) confers resistance to phenylacrylic acids. Cinnamic acid (CA)-sensitive mutants lacking PAD activity were isolated and the PAD1 gene was cloned by phenotypic complementation. The nucleotide sequence of the smallest complementing fragment was determined. The predicted 242-amino-acid PAD polypeptide is 48.6% identical to the product of dedF of Escherichia coli. PAD activity and CA resistance, but not steady-state PAD1 mRNA levels, are influenced by mitochondrial genotype. PAD1 is a single-copy gene in the yeast genome and not essential for viability. The PAD1 locus was physically mapped to a position approx. 140 kb from the left end of chromosome IV.
酵母酶苯丙烯酸脱羧酶（PAD）赋予对苯丙烯酸的抗性。分离出缺乏PAD活性的肉桂酸（CA）敏感突变体，并通过表型互补克隆了PAD1基因。确定了最小互补片段的核苷酸序列。预测的242个氨基酸的PAD多肽与大肠杆菌dedF的产物有48.6%的同一性。PAD活性和对CA的抗性受线粒体基因型影响，但不受稳态PAD1 mRNA水平影响。PAD1是酵母基因组中的单拷贝基因，对生存力并非必需。PAD1基因座在物理图谱上定位于距第四条染色体左端约140 kb的位置。
