几种整合子抗生素抗性基因串联启动子的多样性和相对强度。

Diversity and relative strength of tandem promoters for the antibiotic-resistance genes of several integrons.

作者信息

Lévesque C, Brassard S, Lapointe J, Roy P H

机构信息

Département de Biochimie, Faculté des Sciences et de Génie, Université Laval, Ste-Foy, Québec, Canada.

出版信息

Gene. 1994 May 3;142(1):49-54. doi: 10.1016/0378-1119(94)90353-0.

DOI:10.1016/0378-1119(94)90353-0

PMID:8181756

Abstract

The integron is a new type of mobile element containing one or more antibiotic-resistance-encoding genes site-specifically integrated as cassettes. The integrated genes are expressed from a common promoter region located in an adjacent conserved segment. Sequence analysis has revealed the existence of four versions of the integron promoters. In this study, we have determined the relative strength of the different integron promoters and compared their activity with that of the tac promoter. Each version of the promoter was cloned upstream from a promoter-less chloramphenicol acetyltransferase-encoding gene (cat) in plasmid pKK232-8. CAT activity was used to measure transcriptional expression from the promoters of the antibiotic-resistance operon. The strongest promoter is the version (TTGACAN17TAAACT) found in plasmid R388 and in transposon Tn1696. This promoter is six times more efficient than the derepressed tac promoter.

摘要

整合子是一种新型的可移动元件，含有一个或多个作为盒式结构位点特异性整合的抗生素抗性编码基因。整合的基因从位于相邻保守区段的共同启动子区域表达。序列分析揭示了整合子启动子有四种版本。在本研究中，我们确定了不同整合子启动子的相对强度，并将它们的活性与tac启动子的活性进行了比较。每个启动子版本都克隆到质粒pKK232 - 8中无启动子的氯霉素乙酰转移酶编码基因（cat）的上游。利用氯霉素乙酰转移酶（CAT）活性来测量抗生素抗性操纵子启动子的转录表达。最强的启动子是在质粒R388和转座子Tn1696中发现的版本（TTGACAN17TAAACT）。该启动子的效率比去阻遏的tac启动子高六倍。