Tiemtoré Rahimatou Yasmine Wendkuni, Mètuor Dabiré Amana, Ouermi Djénéba, Sougué Serge, Benao Stéphanie, Simporé Jacques
Biomolecular and Genetic Laboratory (LABIOGENE), Pietro Annigoni Biomolecular Research Center (CERBA), Department of Biochemistry-Microbiology, University Joseph Ki Zerbo, Ouagadougou, Burkina Faso.
Department of Biochemistry-Microbiology, University of Dédougou, Dédougou, Burkina Faso.
Infect Drug Resist. 2022 Jun 20;15:3191-3204. doi: 10.2147/IDR.S360945. eCollection 2022.
Bacterial resistance to beta lactamins is a real public health problem as it complicates treatment strategies. Several types of beta lactamase confer this resistance. Numerous studies report a high prevalence of ESBL producers among Gram-negative bacilli. The objective of this work was to identify the presence of the resistance gene GES in strains of and in Burkina Faso.
During this study 39 strains of and resistant to oxyimino-cephalosporin and monobactam were collected in several samples and analyzed to determine the presence of the beta lactamase resistance gene Bla by classic PCR.
In the present study, resistant strains were observed in 21 E. coli and 18 K. pneumoniae. Among producers of ESBL isolates, the presence of the GES gene was detected up to 63% in and 37% in .
This study highlighted the presence of the GES gene in strains of and resistant to oxyimino-cephalosporin and monobactam in Burkina Faso. This highlights the presence of new ESBL in Burkina, which is of great interest for the proper care of patients and the control of resistance to antibiotics.
细菌对β-内酰胺类抗生素的耐药性是一个切实存在的公共卫生问题，因为它使治疗策略变得复杂。几种类型的β-内酰胺酶赋予了这种耐药性。大量研究报告称革兰氏阴性杆菌中产超广谱β-内酰胺酶（ESBL）的菌株普遍存在。这项工作的目的是确定布基纳法索的大肠杆菌和肺炎克雷伯菌菌株中耐药基因GES的存在情况。
在本研究中，从多个样本中收集了39株对氧亚氨基头孢菌素和单环β-内酰胺耐药的大肠杆菌和肺炎克雷伯菌菌株，并通过经典聚合酶链反应（PCR）分析以确定β-内酰胺酶耐药基因Bla的存在情况。
在本研究中，在21株大肠杆菌和18株肺炎克雷伯菌中观察到耐药菌株。在产ESBL分离株中，在大肠杆菌中检测到GES基因的存在率高达63%，在肺炎克雷伯菌中为37%。
本研究突出了布基纳法索对氧亚氨基头孢菌素和单环β-内酰胺耐药的大肠杆菌和肺炎克雷伯菌菌株中GES基因的存在情况。这凸显了布基纳法索新的ESBL的存在，这对于患者的合理治疗和抗生素耐药性的控制具有重要意义。
