百日咳博德特氏菌超氧化物歧化酶编码基因的鉴定及超氧化物歧化酶缺陷型突变体的构建。
Characterization of the gene encoding superoxide dismutase of Bordetella pertussis and construction of a SOD-deficient mutant.
作者信息
DeShazer D, Bannan J D, Moran M J, Friedman R L
机构信息
Department of Microbiology and Immunology, University of Arizona, Tucson 85724.
出版信息
Gene. 1994 May 3;142(1):85-9. doi: 10.1016/0378-1119(94)90359-x.
The Bordetella pertussis gene sodB, encoding superoxide dismutase (SOD), was cloned by complementation of an Escherichia coli sodAsodB double mutant. The nucleotide sequence of sodB predicted a 21-kDa protein with homology to manganese- and iron-containing SODs from other organisms. Examination of SOD activity on gels suggests that B. pertussis extracts have a single SOD containing Fe3+ as a prosthetic group. A SOD-deficient mutant was obtained by insertional inactivation of sodB in B. pertussis, confirming that there is only one SOD in this organism.
通过对大肠杆菌sodAsodB双突变体进行互补，克隆出了编码超氧化物歧化酶（SOD）的百日咳博德特氏菌基因sodB。sodB的核苷酸序列预测其编码一种21 kDa的蛋白质，该蛋白质与其他生物体中含锰和含铁的SOD具有同源性。凝胶上SOD活性检测表明，百日咳博德特氏菌提取物有一种以Fe3+作为辅基的单一SOD。通过在百日咳博德特氏菌中插入失活sodB获得了一个SOD缺陷型突变体，证实该生物体中只有一种SOD。
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