Johansson M, Dietrich C, Mandahl N, Hambraeus G, Johansson L, Clausen P P, Mitelman F, Heim S
Department of Clinical Genetics, Lund University Hospital, Sweden.
Int J Cancer. 1994 May 15;57(4):463-7. doi: 10.1002/ijc.2910570404.
Cytogenetic analysis of short-term cultures from 26 primary bronchial large cell carcinomas and 1 metastasis from a primary large cell carcinoma revealed clonal chromosome abnormalities in 20 tumors and a normal karyotype in 6. No outgrowth was obtained in 1 case. Simple aberrations were present in 3 tumors; in 1, the only change was a reciprocal translocation between chromosomes 1 and 6, in another the sole anomaly was a supernumerary marker ring chromosome and in a third loss of the Y chromosome was the only clonal change. The remaining 17 tumors had complex karyotypes. The chromosomes most frequently involved in structural rearrangements were chromosomes 1, 2, 3, 5, 6, 7, 8, 10, 11, 13, 14 and 17. The bands most frequently affected were 1q11-12, 1p13, 7q11, and 17p11-13. The rearrangements led to repeated losses of 1p, 1q, 3p, 6q, 7q and 17p and gains of 5q and 7p. The emerging karyotypic picture of large cell lung carcinomas indicates more similarities with adenocarcinomas than with other pathologic subgroups of lung cancer.
对26例原发性支气管大细胞癌的短期培养物及1例原发性大细胞癌转移灶进行细胞遗传学分析，结果显示20例肿瘤存在克隆性染色体异常，6例核型正常。1例未获得细胞生长。3例肿瘤存在简单畸变；1例中唯一的改变是1号和6号染色体之间的相互易位，另一例唯一的异常是一条额外的标记环形染色体，第三例中唯一的克隆性改变是Y染色体缺失。其余17例肿瘤具有复杂核型。结构重排中最常涉及的染色体是1、2、3、5、6、7、8、10、11、13、14和17号染色体。最常受影响的带区是1q11 - 12、1p13、7q11和17p11 - 13。这些重排导致1p、1q、3p、6q、7q和17p反复缺失以及5q和7p增加。大细胞肺癌新出现的核型图景表明，与腺癌的相似性高于与肺癌其他病理亚组的相似性。
