Alenius H, Turjanmaa K, Mäkinen-Kiljunen S, Reunala T, Palosuo T
Department of Immunobiology, National Public Health Institute, Helsinki, Finland.
J Allergy Clin Immunol. 1994 May;93(5):859-63. doi: 10.1016/0091-6749(94)90378-6.
In the present immunoblot study we examined IgE immune responses to natural rubber latex proteins in sera from 27 adult patients with latex allergy. We demonstrated at least 30 proteins in the rubber tree sap, of which 19 bound IgE antibodies in a heterogeneous manner in patient sera. Twenty-two of the 27 patient sera (81%) showed positive immunoblot test results. IgE antibodies in the patient sera bound most frequently to a 20 kd allergen, which was recognized by 17 of the 22 (77%) patient sera with positive immunoblot test results. These results suggest that a 20 kd rubber protein could be a major allergen in adult patients with latex allergy. Immunoblotting with a representative set of allergens as antigen would offer a sensitive, confirmatory in vitro test to demonstrate hypersensitivity to latex.
在本免疫印迹研究中，我们检测了27名成年乳胶过敏患者血清中针对天然橡胶乳胶蛋白的IgE免疫反应。我们证实橡胶树汁液中至少有30种蛋白质，其中19种在患者血清中以异质性方式结合IgE抗体。27名患者血清中有22份（81%）免疫印迹检测结果呈阳性。患者血清中的IgE抗体最常与一种20kd的过敏原结合，在免疫印迹检测结果呈阳性的22份（77%）患者血清中，有17份可识别该过敏原。这些结果表明，一种20kd的橡胶蛋白可能是成年乳胶过敏患者的主要过敏原。用一组代表性过敏原作为抗原进行免疫印迹，将提供一种敏感的体外确证试验，以证明对乳胶过敏。
