Szépfalusi Z, Ebner C, Pandjaitan R, Orlicek F, Scheiner O, Boltz-Nitulescu G, Kraft D, Ebner H
Institute of General and Experimental Pathology, Vienna, Austria.
J Allergy Clin Immunol. 1994 May;93(5):932-42. doi: 10.1016/0091-6749(94)90388-3.
Thirty-one patients with clinical history of egg allergy, bird allergy, or bird and egg allergy were investigated with the use of the immunoblot technique to compare IgE-binding components in bird feather and egg yolk and white extracts. Patients were classified into three groups according to clinical history, skin prick test results, and RAST results. Patients in group I were sensitized to bird feathers and egg yolk, patients in group II to egg white, and patients in group III to bird feather but not to eggs. Patients with bird-egg syndrome were mainly female adults, whereas egg white allergy was mainly observed in children without any obvious sex predisposition. IgE from patients with bird-egg syndrome recognized a 70 kd protein in egg yolk (chicken serum albumin = alpha-livetin) and some major allergens in bird feather extract (70, 95, and 200 kd). Preincubation of pooled sera from patients with bird-egg syndrome with budgerigar or hen feather extract and egg yolk extract, respectively, led to complete blocking of IgE binding to allergens in egg yolk and bird feather extract. On the other hand, IgE from patients with egg white allergy did not react with allergens in egg yolk and bird feather extract, despite strong IgE binding to egg white allergens. Patients in group III displayed no reactivity to bird feather or egg allergens. Our results demonstrate common epitopes of budgerigar and hen feather and egg yolk alpha-livetin. Therefore we assume that alpha-livetin (chicken serum albumin) leads to a cross-sensitization and consequently to the "bird-egg syndrome."
采用免疫印迹技术对31例有鸡蛋过敏、鸟类过敏或鸟类和鸡蛋过敏临床病史的患者进行研究，以比较鸟类羽毛、蛋黄和蛋清提取物中的IgE结合成分。根据临床病史、皮肤点刺试验结果和RAST结果将患者分为三组。第一组患者对鸟类羽毛和蛋黄过敏，第二组对蛋清过敏，第三组对鸟类羽毛过敏但对鸡蛋不过敏。鸟蛋综合征患者主要为成年女性，而蛋清过敏主要见于儿童，无明显性别倾向。鸟蛋综合征患者的IgE识别蛋黄中的一种70kd蛋白（鸡血清白蛋白=α-卵黄磷蛋白）和鸟类羽毛提取物中的一些主要过敏原（70、95和200kd）。分别用虎皮鹦鹉或母鸡羽毛提取物和蛋黄提取物对鸟蛋综合征患者的混合血清进行预孵育，可完全阻断IgE与蛋黄和鸟类羽毛提取物中过敏原的结合。另一方面，蛋清过敏患者的IgE尽管与蛋清过敏原强烈结合，但不与蛋黄和鸟类羽毛提取物中的过敏原发生反应。第三组患者对鸟类羽毛或鸡蛋过敏原无反应。我们的结果证明了虎皮鹦鹉和母鸡羽毛以及蛋黄α-卵黄磷蛋白的共同表位。因此，我们推测α-卵黄磷蛋白（鸡血清白蛋白）会导致交叉致敏，进而导致“鸟蛋综合征”。
