枯草芽孢杆菌脂肪酶的结晶及X射线初步分析

Crystallization and preliminary X-ray analysis of a lipase from Bacillus subtilis.

作者信息

Ransac S, Blaauw M, Lesuisse E, Schanck K, Colson C, Dijkstra B W

机构信息

Laboratory of Biophysical Chemistry, University of Groningen, The Netherlands.

出版信息

J Mol Biol. 1994 May 20;238(5):857-9. doi: 10.1006/jmbi.1994.1342.

DOI:10.1006/jmbi.1994.1342

PMID:8182756

Abstract

Single crystals of the lipase from Bacillus subtilis have been obtained using a mixture of polyethylene glycol 4000 and sodium sulphate solution as the precipitant. The crystals grow at room temperature in two to three weeks in the presence of n-octyl-beta-D-glucoside. They belong to the monoclinic space group C2 with a = 121.20 A, b = 93.19 A, c = 80.96 A, and beta = 110.67 degrees, with four protein molecules per asymmetric unit. The crystals diffract to at least 2.5 A resolution and are suitable for an X-ray structure analysis.

摘要

使用聚乙二醇4000和硫酸钠溶液的混合物作为沉淀剂，已获得枯草芽孢杆菌脂肪酶的单晶。晶体在正辛基-β-D-葡萄糖苷存在下于室温下生长两到三周。它们属于单斜空间群C2，a = 121.20 Å，b = 93.19 Å，c = 80.96 Å，β = 110.67°，每个不对称单元有四个蛋白质分子。这些晶体的衍射分辨率至少为2.5 Å，适合进行X射线结构分析。