Sorrell M J, Tribble J, Reinisch L, Werkhaven J A, Ossoff R H
Department of Otolaryngology, Vanderbilt University Medical Center, Nashville, Tennessee 37232.
Lasers Surg Med. 1994;14(2):155-63. doi: 10.1002/1096-9101(1994)14:2<155::aid-lsm1900140208>3.0.co;2-l.
We have investigated the fluorescence profiles of four common pathogens: S. pneumoniae, S. aureus, M. catarrhalis, and H. influenzae. The steady-state auto fluorescence spectra of bacteria are measured as a function of the incident light from 200 to 700 nm. The spectra for each bacterium are combined into a fluorescence profile or fluorescence finger print. Each bacterium produces a unique in vitro fluorescence profile when measured in a saline suspension. The profiles are reproducible. Suspensions of a bacterial strain, where the identification is not known, can be correctly matched to a small library of previously measured fluorescence profiles using a linear least-squares fitting algorithm. In addition, we have measured the fluorescence and absorption spectrum of the tympanic membrane removed from a chinchilla. The optical properties of the tympanic membrane and the least-squares identification process form precept for a non-invasive, fluorescence based bacterial diagnosis technique to be used in otitis media.
肺炎链球菌、金黄色葡萄球菌、卡他莫拉菌和流感嗜血杆菌。测量细菌的稳态自发荧光光谱，作为入射光在200至700纳米范围内的函数。每种细菌的光谱被组合成一个荧光图谱或荧光指纹。当在盐溶液悬浮液中测量时，每种细菌都会产生独特的体外荧光图谱。这些图谱是可重复的。对于一个未知鉴定结果的细菌菌株悬浮液，可以使用线性最小二乘法拟合算法将其与一个先前测量的荧光图谱小文库进行正确匹配。此外，我们还测量了从龙猫身上取下的鼓膜的荧光和吸收光谱。鼓膜的光学特性和最小二乘法鉴定过程为用于中耳炎的非侵入性荧光细菌诊断技术奠定了基础。
