Smirnova N I, Davydova N I, Livanova L F
Mol Gen Mikrobiol Virusol. 1994 Mar-Apr(2):25-8.
A new mutation tox-2 defining the increased level of cholera exotoxin production by the strain Vibrio cholerae Dakka 35 isolated from nature has been mapped by conjugational crosses of donor and recipient strains differing by toxin production and serovar. The mutation has been localized on the chromosomal fragment containing the ilv, pur, ura, rfb genes adjacent to ura-94 locus. The linkage of the tox-2 mutation with the rfb locus coding for the synthesis of somatic Ol-antigen has been also established.
通过对产毒素和血清型不同的供体菌株与受体菌株进行接合杂交，定位了一种新的突变体tox-2，该突变体决定了从自然界分离出的霍乱弧菌达卡35菌株霍乱毒素产生水平的提高。该突变已定位在含有ilv、pur、ura、rfb基因的染色体片段上，这些基因与ura-94位点相邻。还确定了tox-2突变与编码体细胞O1抗原合成的rfb位点之间的连锁关系。
