Agalykov N R, Kullyev P K, Matvienko N I
Mol Gen Mikrobiol Virusol. 1994 Mar-Apr(2):3-7.
The restriction map of the nuclear polyhedrosis DNA virus of Bombyx mori has been determined with the use of four restriction endonucleases: NotI, BamHI, SmaI and KpnI. 18 cleavage sites have been localized on the viral DNA. Total length of the viral DNA is about 130 kb. The fragment containing a polyhedrin gene has been localized on the map by the blot hybridization technique.
利用四种限制性内切酶NotI、BamHI、SmaI和KpnI确定了家蚕核型多角体DNA病毒的限制酶切图谱。已在病毒DNA上定位了18个切割位点。病毒DNA的总长度约为130kb。通过印迹杂交技术已在图谱上定位了包含多角体蛋白基因的片段。