家蚕核型多角体病毒基因组的黏粒文库构建及物理图谱绘制
Construction of a cosmid library and physical mapping of Bombyx mori nucleopolyhedrovirus genome.
作者信息
Shikata M, Hashimoto Y, Sano Y, Matsumoto T
机构信息
Department of Applied Biology, Kyoto Institute of Technology, Japan.
出版信息
New Microbiol. 1998 Jul;21(3):259-62.
Bombyx mori nucleopolyhedrovirus (BmNPV) DNA was partially digested with BamHI and BglII, or EcoRI, and cloned into the cosmid pWE15. Eight cosmid clones possessing viral DNA fragments with a size range from 34.9 kb to 45.1 kb were obtained, and they covered the entire viral DNA sequence. Using the cosmid library and plasmid libraries obtained previously, physical maps of BmNPV DNA were constructed for EcoRI, SmaI, PstI, SacI and SacII.
用BamHI和BglII或EcoRI对家蚕核型多角体病毒（BmNPV）DNA进行部分酶切，并克隆到黏粒pWE15中。获得了8个含有大小范围从34.9 kb到45.1 kb病毒DNA片段的黏粒克隆，它们覆盖了整个病毒DNA序列。利用先前获得的黏粒文库和质粒文库，构建了BmNPV DNA的EcoRI、SmaI、PstI、SacI和SacII物理图谱。
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