Maeda T, Wurgler-Murphy S M, Saito H
Division of Tumor Immunology, Dana-Farber Cancer Institute, Boston, Massachusetts.
Nature. 1994 May 19;369(6477):242-5. doi: 10.1038/369242a0.
In the prokaryotic two-component signal transduction systems, recognition of an environmental stimulus by a sensor molecule results in the activation of its histidine kinase domain and phosphorylation of a histidine residue within that domain. This phosphate group is then transferred to an aspartate residue in the receiver domain of a cognate response regulator molecule, resulting in the activation of its output function. Although a few eukaryotic proteins were identified recently that show sequence similarity to the prokaryotic sensors or response regulators, it has not been clear whether they constituted a part of a 'two-component' system. Here we describe a two-component system in Saccharomyces cerevisiae that regulates an osmosensing MAP kinase cascade.
在原核生物双组分信号转导系统中，传感器分子对环境刺激的识别会导致其组氨酸激酶结构域的激活以及该结构域内一个组氨酸残基的磷酸化。然后，这个磷酸基团会转移至同源应答调节分子的接收结构域中的一个天冬氨酸残基上，从而激活其输出功能。尽管最近发现了一些与原核生物传感器或应答调节因子具有序列相似性的真核生物蛋白质，但它们是否构成“双组分”系统的一部分尚不清楚。在此，我们描述了酿酒酵母中一个调节渗透感应丝裂原活化蛋白激酶级联反应的双组分系统。
