Minamoto A, Sakata H, Tsumura K, Yamamoto M, Kusunoki S
Department of Ophthalmology, Hiroshima University School of Medicine, Japan.
Ophthalmologica. 1994;208(2):105-9. doi: 10.1159/000310462.
We used a rat model of orthotopic penetrating keratoplasty to study splenic cell cytotoxicity in the host. DA (RT1avl) rats received grafts of Fischer (F344, RT1lv1) rat corneas. Cytotoxic T lymphocyte (CTL) activity was measured by the cell-mediated lymphocytotoxicity assay using 4/4R.M.-4 cells as target cells. CTL activity became detectable in vitro 2 weeks after grafting and peaked at 3 weeks. This simple method of quantifying CTL activity in the orthotopic penetrating keratoplasty model should provide a reliable tool for studying the CTL response in corneal transplantation.
我们使用原位穿透性角膜移植大鼠模型来研究宿主中的脾细胞细胞毒性。DA（RT1avl）大鼠接受了Fischer（F344，RT1lv1）大鼠角膜移植。使用4/4R.M.-4细胞作为靶细胞，通过细胞介导的淋巴细胞毒性试验测量细胞毒性T淋巴细胞（CTL）活性。移植后2周可在体外检测到CTL活性，并在3周时达到峰值。这种在原位穿透性角膜移植模型中量化CTL活性的简单方法应为研究角膜移植中的CTL反应提供可靠工具。
