Yutani Y, Inui K, Konishi H, Kitano T, Hayashi S, Asada K, Yamano Y
Department of Orthopaedics, Osaka City University Medical School, Japan.
Osaka City Med J. 1993 Nov;39(2):67-74.
Human cartilaginous cells produce two types of proteoglycan monomers (PGI and PGII) distinguishable by their molecular size in density gradient centrifugation under dissociative conditions. In order to determine whether connective tissues on the sliding surface of the acetabulum on an endoprosthesis are able to differentiate into cartilaginous tissue, we made histological and biochemical analysis of the incorporation of 35S and the distribution of the molecular size of proteoglycans produced. Histologically, dense collagen fibers were observed both on weight-bearing and non-weight-bearing surfaces, but there was little evidence of cartilaginous metaplasia. Biochemical analysis, however, demonstrated that incorporation of 35S in newly synthesized glycosaminoglycan (GAG) was higher than that in non-cartilaginous tissues, suggesting that cartilaginous differentiation of mesenchymal tissues had occurred.
人类软骨细胞产生两种蛋白聚糖单体（PGI和PGII），在解离条件下通过密度梯度离心可根据其分子大小区分。为了确定人工关节髋臼滑动面上的结缔组织是否能够分化为软骨组织，我们对35S的掺入以及所产生蛋白聚糖的分子大小分布进行了组织学和生化分析。组织学上，在负重面和非负重面上均观察到致密的胶原纤维，但几乎没有软骨化生的证据。然而，生化分析表明，新合成的糖胺聚糖（GAG）中35S的掺入高于非软骨组织，这表明间充质组织发生了软骨分化。
