Yutani Y, Yamano Y
Department of Orthopedics Osaka City University Medical School, Japan.
Osaka City Med J. 1996 Jul;42(1):37-44.
Articular cartilage consists of chondrocytes and a cartilagenous matrix produced by these cells. The cartilaginous matrix is considered to undergo various changes in response to the surrounding environment. In this study, cartilaginous changes related to osteoarthritis of the knee were investigated. Histological changes were apparent in medical joint lesions, and changes typical of osteoarthritis, such as cell clustering and disruption of the matrix, were always observed. Degeneration was less severe in the lateral joint lesions. A biochemical analysis was undertaken and the molecular size of the proteoglycan produced in the matrix was measured by glycerol density gradient ultracentrifugation method, following pulse labeling with 35S-Sulfuric acid. The proteoglycan produced in the histologically altered regions tended to be of low molecular size. However, the size was still significantly larger than that of proteoglycans located in the nonarticular tissues such as synovia membrane, so the differentiated phenotype of chondrocytes persisted.
关节软骨由软骨细胞和这些细胞产生的软骨基质组成。软骨基质被认为会根据周围环境发生各种变化。在本研究中，对与膝关节骨关节炎相关的软骨变化进行了调查。在医学关节病变中组织学变化明显，并且总是观察到骨关节炎的典型变化，如细胞聚集和基质破坏。外侧关节病变的退变较轻。进行了生化分析，并在使用35S-硫酸进行脉冲标记后，通过甘油密度梯度超速离心法测量了基质中产生的蛋白聚糖的分子大小。组织学改变区域产生的蛋白聚糖往往分子量较小。然而，其大小仍明显大于位于非关节组织如滑膜中的蛋白聚糖，因此软骨细胞的分化表型得以持续。
