Liang T, Raugi G J, Blum J J
J Protozool. 1976 Feb;23(1):186-93. doi: 10.1111/j.1550-7408.1976.tb05268.x.
The growth of Tetrahymena pyriformis strain HSM was strongly inhibited by 4-pentenoic acid. Supplementing the medium acetate reversed the growth inhibition, but pyruvate was ineffective. Glycogen content was much lower in cells grown with 4-pentenoic acid than in controls; this effect was not reversed by acetate or by pyruvate. There was little effect of 4-penteonic acid on the in incorporation of label from [1-14C]acetate, [2-14C]glycerol, [1-14C]ribose, [U-14C]fructose, or [1-14C]glucose into CO2 but incorporation of label into glycogen was inhibited, the strongest inhibition being on acetate and the weakest approximately 20%) on ribose, fructose, and glucose. A 3-compartment model for quantitation of labeled acetyl CoA fluxes was shown to be applicable to Tetrahymena grown in the presence of 4-pentenoic acid, and experiments were performed to establish the flux of [1-14C]acetyl CoA into glycogen, lipids, CO2, glutamate, and alanine. It was evident from the results of these experiments that 4-pentenoic acid did not appreciably inhibit beta-oxidation or lipogenesis, but markedly decreased the glyconeogenic flux of labeled acetyl-CoA from the peroxismal and outer mitochondrial compartments.
4-戊烯酸强烈抑制梨形四膜虫HSM株的生长。在培养基中添加乙酸盐可逆转生长抑制作用，但丙酮酸盐无效。与对照相比，在含有4-戊烯酸的培养基中生长的细胞中糖原含量要低得多；乙酸盐或丙酮酸盐都不能逆转这种效应。4-戊烯酸对[1-¹⁴C]乙酸盐、[2-¹⁴C]甘油、[1-¹⁴C]核糖、[U-¹⁴C]果糖或[1-¹⁴C]葡萄糖中标记物掺入二氧化碳的过程影响很小，但标记物掺入糖原的过程受到抑制，对乙酸盐的抑制作用最强，对核糖、果糖和葡萄糖的抑制作用最弱（约20%）。一个用于定量标记乙酰辅酶A通量的三室模型被证明适用于在4-戊烯酸存在下生长的四膜虫，并进行了实验以确定[1-¹⁴C]乙酰辅酶A进入糖原、脂质、二氧化碳、谷氨酸和丙氨酸的通量。从这些实验结果可以明显看出，4-戊烯酸并未明显抑制β-氧化或脂肪生成，但显著降低了过氧化物酶体和线粒体外膜室中标记乙酰辅酶A的糖异生通量。
