Rychlík I, Kubícek O, Holcák V, Bárta J, Pavlík I
Veterinary Research Institute, Brno.
Vet Med (Praha). 1994;39(2-3):111-6.
This paper describes the use of the oligonucleotide probe (GTG)5 to reveal high polymorphic DNA regions in falcons (Falco peregrinus, F. rusticolus, F. cherrug and their interspecies hybrids). Ten microliters of the blood samples were immobilized, lysed and digested in low-melting point agarose. Oligonucleotide probe (GTG)5 gave rise to the great number of different fragments. Some of them were genus specific, another female specific and approx. 5-10% of the fragments were individual specific. Restriction endonucleases with 4 bp recognition sequences were preferred (Hinf I, Hae III and Msp I). After the use of such enzymes the DNA fingerprints were individual specific and allowed us to confirm known relations among individual birds. The results indicate, that DNA fingerprinting with oligonucleotide (GTG)5 as a probe could be a powerful method for differentiating among closely related falcon birds.
本文描述了使用寡核苷酸探针(GTG)5来揭示猎鹰(矛隼、矛斑隼、猎隼及其种间杂种)中高度多态的DNA区域。将10微升血样固定在低熔点琼脂糖中进行裂解和消化。寡核苷酸探针(GTG)5产生了大量不同的片段。其中一些片段是属特异性的，另一些是雌性特异性的，约5 - 10%的片段是个体特异性的。优先使用具有4个碱基识别序列的限制性内切酶(Hinf I、Hae III和Msp I)。使用这些酶后，DNA指纹具有个体特异性，使我们能够确认个体鸟类之间已知的亲缘关系。结果表明，以寡核苷酸(GTG)5为探针的DNA指纹图谱可能是区分亲缘关系密切的猎鹰的一种有效方法。
