Zaitlin M, Anderson J M, Perry K L, Zhang L, Palukaitis P
Department of Plant Pathology, Cornell University, Ithaca, New York 14853.
Virology. 1994 Jun;201(2):200-5. doi: 10.1006/viro.1994.1286.
Plants transformed with a nucleotide sequence coding for a truncated RNA 2 replicase gene of the subgroup I strain of cucumber mosaic virus, Fny-CMV, are resistant to cucumber mosaic disease. Two resistant lines representing independent transformations in the original study have been propagated, and their progeny have been examined. Resistance to Fny-CMV was genetically integrated and was retained in the R4 generation. Fourteen subgroup I CMV strains, 6 strains uncharacterized as to subgroup, and 4 subgroup II CMV strains, as well as potato virus X, tobacco mosaic virus, tomato aspermy virus, tobacco etch virus, and peanut stunt virus were inoculated mechanically to R2 generation plants of these two lines. Resistance was found to 12 subgroup I CMV strains and to all of the subgroup-uncharacterized strains. No resistance was found to subgroup II CMV strains nor to the other viruses listed above. However, the two plant lines behaved differently in response to 3 subgroup I strains, K-CMV, PRC-CMV, and MB-CMV, where one line (5) was more resistant than the other (2). RNase protection analyses indicated that these 3 strains vary more in sequence from Fny-CMV than do the other subgroup I strains tested. Resistance was also shown when aphids were used to inoculate plants with either of 2 subgroup I CMV strains; no resistance was observed when aphids were used to transmit a subgroup II CMV strain. Inoculation of transgenic plants with pseudorecombinants produced with the RNAs derived from Fny-CMV to which the plants are resistant, and LS-CMV, a subgroup II strain to which they are susceptible, indicated that the resistance-breaking capacity of LS-CMV is a function of RNA 2.
用编码黄瓜花叶病毒I亚组Fny - CMV株系截短的RNA 2复制酶基因的核苷酸序列转化的植物，对黄瓜花叶病具有抗性。在最初的研究中，代表独立转化的两个抗性株系已进行繁殖，并对其后代进行了检测。对Fny - CMV的抗性已整合到基因组中，并在R4代中得以保留。将14个I亚组CMV株系、6个未确定亚组的株系、4个II亚组CMV株系，以及马铃薯X病毒、烟草花叶病毒、番茄不孕病毒、烟草蚀纹病毒和花生矮化病毒机械接种到这两个株系的R2代植株上。发现对12个I亚组CMV株系和所有未确定亚组的株系具有抗性。对II亚组CMV株系和上述其他病毒均未发现抗性。然而，这两个株系对3个I亚组株系K - CMV、PRC - CMV和MB - CMV的反应不同，其中一个株系（5号）比另一个株系（2号）更具抗性。核糖核酸酶保护分析表明，这3个株系与Fny - CMV的序列差异比其他测试的I亚组株系更大。当用蚜虫接种2个I亚组CMV株系中的任何一个时，也表现出抗性；当用蚜虫传播II亚组CMV株系时，未观察到抗性。用对转基因植物具有抗性的Fny - CMV来源的RNA与它们敏感的II亚组株系LS - CMV产生的假重组体接种转基因植物，表明LS - CMV的抗性突破能力是RNA 2的一个功能。
