在澳大利亚感染甘蔗的八种甘蔗花叶马铃薯Y病毒分离株的NIb编码区的序列多样性。

Sequence diversity in the NIb coding region of eight sugarcane mosaic potyvirus isolates infecting sugarcane in Australia.

作者信息

Handley J A, Smith G R, Dale J L, Harding R M

机构信息

Centre for Molecular Biotechnology, School of Life Science, Queensland University of Technology, Brisbane, Australia.

出版信息

Arch Virol. 1996;141(12):2289-300. doi: 10.1007/BF01718631.

DOI:10.1007/BF01718631

PMID:9526537

Abstract

We have sequenced the NIb coding region of sugarcane mosaic potyvirus strain SC (SCMV-SC) and eight field isolates of SCMV from Australia. This region comprised 1563 nucleotides and encoded a putative protein of 521 amino acids containing the consensus motif GDD. The protease cleavage sites between the NIa/NIb and the NIb/coat protein were found to be Q/C and Q/A, respectively. The SCMV sequences were most similar to sorghum mosaic potyvirus with identities of 70% and 78% at the nucleotide and amino acid levels, respectively. When the sequences were compared to each other, there was a maximum of 3.3% variation between isolates at the nucleotide level and a maximum of 0.8% at the amino acid level. Phylogenetic analysis of the sequences indicated the field isolates were grouped according to their geographical location. The SCMV sequence with most homology to all other isolates has been selected to generate constructs for replicase-mediated resistance.

摘要

我们已对甘蔗花叶马铃薯Y病毒SC株系（SCMV-SC）以及来自澳大利亚的8个SCMV田间分离株的NIb编码区进行了测序。该区域由1563个核苷酸组成，编码一个推定的含有共有基序GDD的521个氨基酸的蛋白质。发现NIa/NIb和NIb/外壳蛋白之间的蛋白酶切割位点分别为Q/C和Q/A。SCMV序列与高粱花叶马铃薯Y病毒最为相似，在核苷酸和氨基酸水平上的同一性分别为70%和78%。当将这些序列相互比较时，分离株之间在核苷酸水平上的最大变异为3.3%，在氨基酸水平上的最大变异为0.8%。序列的系统发育分析表明，田间分离株根据其地理位置进行分组。已选择与所有其他分离株具有最高同源性的SCMV序列来构建用于复制酶介导抗性的构建体。