Centre Hospitalier Universitaire de Nice, Unité de Thérapie Cellulaire et Génique, Nice, France.
Université Côte d'Azur, EA 7354 MICORALIS, UFR Odontologie, Nice, France.
Curr Res Transl Med. 2019 Aug;67(3):107-111. doi: 10.1016/j.retram.2019.03.003. Epub 2019 Mar 29.
In ABO-incompatible bone marrow transplantation, an efficient depletion of red blood cells (RBC) within the graft is mandatory to avoid adverse events in transplanted patients. Using non therapeutic products, we evaluated the substitution of the standard density gradient-based separation (DGBS) over Ficoll-Paque with the use of an automated procedure intended for buffy coat only (SmartRedux software) introducing modifications within the settings to achieve a drastic reduction of the initial volume of the product. Both methods were conducted on the Sepax-2 device.
RBC depletion rates and CD34+ cells recoveries from eight procedures with SmartRedux software using "in-house" settings (method A) were compared to those obtained from four procedures using NeatCell software, an automated DGBS over Ficoll-Paque (method B).
Median erythrocyte depletion of 95,4% (92,7%-99,0%) and 99,8% (99,0%-99,9%) were observed using methods A and B, respectively. Median residual RBC volumes in the final product were 19 mL (4,4 mL-31,2 mL) and 0,7 mL (0,4 mL-4,7 mL), respectively (p = 0,014). CD34+ cells recoveries of 90,9% (62,7%-102,1%) and 78,4% (64,1%-86,2%) were achieved for methods A and B. Median platelet depletion was 16,6% (10%-42,7%) and 89,8% (88,5%-92,4%) using methods A and B, respectively (p = 0,004). Processing duration was shorter using method A (168 ± 29 min) than method B (295 ± 21 min) (p = 0,004).
Both methods achieved satisfactory erythrocyte depletion and CD34+ recovery. The use of Sepax-2 device in association with SmartRedux software could be extended to efficiently deplete RBC from large-volume BM in a raw instead of DGBS.
在 ABO 不相容骨髓移植中，必须有效地去除移植物中的红细胞（RBC），以避免移植患者发生不良反应。我们使用非治疗性产品，评估了使用仅用于白细胞层的自动化程序替代标准密度梯度分离（DGBS）的效果（SmartRedux 软件），并在设置中引入了一些修改，以大幅度减少产品的初始体积。两种方法都在 Sepax-2 设备上进行。
比较了使用 SmartRedux 软件的“内部”设置（方法 A）进行的 8 个程序与使用 NeatCell 软件（Ficoll-Paque 上的自动 DGBS）进行的 4 个程序的 RBC 去除率和 CD34+细胞回收率（方法 B）。
使用方法 A 和 B 分别观察到红细胞去除率中位数为 95.4%（92.7%-99.0%）和 99.8%（99.0%-99.9%）。最终产品中残留 RBC 体积中位数分别为 19ml（4,4ml-31,2ml）和 0.7ml（0.4ml-4.7ml）（p=0.014）。方法 A 和 B 的 CD34+细胞回收率分别为 90.9%（62.7%-102.1%）和 78.4%（64.1%-86.2%）。使用方法 A 和 B 时，血小板去除率中位数分别为 16.6%（10%-42.7%）和 89.8%（88.5%-92.4%）（p=0.004）。方法 A（168±29 分钟）的处理时间短于方法 B（295±21 分钟）（p=0.004）。
两种方法均能达到满意的红细胞去除率和 CD34+回收率。Sepax-2 设备与 SmartRedux 软件联合使用可扩展用于从大容量骨髓中有效去除 RBC，而无需进行 DGBS。
