通过酶联免疫吸附测定法检测感染牛瘟病毒的家兔中的抗DNA抗体反应。
Detection of anti-DNA antibody responses in rabbits infected with rinderpest virus by ELISA.
作者信息
Rattan B, Sharma B, Bansal R P
机构信息
Division of Virology, Indian Veterinary Research Institute, Mukteswar-Kumaon, Nainital.
出版信息
Acta Virol. 1993 Oct;37(5):403-6.
ELISA was standardized to detect anti-DNA antibodies (ADAbs) in sera of rabbits infected with rinderpest virus (RPV). These antibodies were found in late (28th and 36th day post inoculation (dpi)) sera of rabbits infected with rabbit virulent lapinized RPV, but not in early (7th dpi) sera of the same rabbits. ADAbs were not found either in early or late sera of rabbits infected with attenuated RBOK strain of RPV. Rabbit anti-RPV hyperimmune serum was also found to be positive for ADAbs. Detection of these antibodies by ELISA proved DNA to be one of the target antigens for antinuclear antibodies induced in RPV infection.
酶联免疫吸附测定（ELISA）经过标准化处理，用于检测感染牛瘟病毒（RPV）的家兔血清中的抗DNA抗体（ADAbs）。这些抗体在感染兔强毒兔化牛瘟病毒的家兔的晚期（接种后第28天和第36天）血清中被发现，但在同一批家兔的早期（接种后第7天）血清中未被发现。在感染牛瘟病毒减毒RBOK株的家兔的早期或晚期血清中均未发现抗DNA抗体。兔抗牛瘟病毒超免疫血清也被发现抗DNA抗体呈阳性。通过ELISA检测这些抗体证明DNA是牛瘟病毒感染诱导产生的抗核抗体的靶抗原之一。
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