感染牛瘟病毒的兔子对双链DNA和核组蛋白抗原的免疫耐受性暂时丧失。
Temporary breakdown of immunological tolerance to dsDNA and nucleohistone antigens in rabbits infected with rinderpest virus.
作者信息
Imaoka K, Kanai Y, Yoshikawa Y, Yamanouchi K
机构信息
Laboratory Animal Research Centre, University of Tokyo, Japan.
出版信息
Clin Exp Immunol. 1990 Dec;82(3):522-6. doi: 10.1111/j.1365-2249.1990.tb05483.x.
The rabbit-passaged L strain of rinderpest virus (RV) causes the transient induction of anti-nuclear antibodies (ANA) in rabbits. It has been shown by an indirect immunofluorescence test that the target antigens of these ANA are DNA and/or DNA-histone complexes (nucleohistone). Here detailed examinations of the target antigens were carried out by ELISA, and it was revealed that rabbit sera contained three types of antibodies: antibodies reacting equally with both dsDNA and ssDNA; those reacting with ssDNA alone; and those reacting with nucleohistone. Epitopes recognized by the third type consisted of complexes of dsDNA and H2A + H2B or of dsDNA and H2B. All types of antibodies were antigen specific. Since the diversity of ANA among experimental rabbits was large, it was suggested that genetic background is important in the induction of anti-dsDNA antibodies in this system. Moreover, early induction of antibodies to nucleohistone and the rapid disappearance of ANA suggest that B cell proliferation/maturation for continuous production of ANA requires factors other than RV infection. This system may help elucidate the mechanisms of ANA induction and the development of autoimmune diseases.
经兔传代的牛瘟病毒（RV）L株可在兔体内短暂诱导抗核抗体（ANA）产生。间接免疫荧光试验表明，这些ANA的靶抗原是DNA和/或DNA-组蛋白复合物（核组蛋白）。在此，通过酶联免疫吸附测定（ELISA）对靶抗原进行了详细检测，结果显示兔血清中含有三种抗体：与双链DNA（dsDNA）和单链DNA（ssDNA）均能同等反应的抗体；仅与ssDNA反应的抗体；以及与核组蛋白反应的抗体。第三类抗体识别的表位由dsDNA与H2A + H2B的复合物或dsDNA与H2B的复合物组成。所有类型的抗体均具有抗原特异性。由于实验兔之间ANA的多样性较大，提示在该系统中，遗传背景对于抗dsDNA抗体的诱导很重要。此外，针对核组蛋白抗体的早期诱导以及ANA的快速消失表明，持续产生ANA的B细胞增殖/成熟需要除RV感染之外的其他因素。该系统可能有助于阐明ANA诱导机制及自身免疫性疾病的发展过程。
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