Gerlach J, Schnoy N, Smith M D, Neuhaus P
Chirurgische Klinik, Universitätsklinikum Rudolf Virchow, Freie Universität Berlin, Germany.
Artif Organs. 1994 Mar;18(3):226-30. doi: 10.1111/j.1525-1594.1994.tb02181.x.
A multi-compartment capillary membrane culture model with independently perfused three-dimensionally woven capillaries was developed for immobilization of hepatocytes in bioreactors. This enables spatial restructuring of cells and enhanced mass transfer performance with more efficient oxygenation and metabolite exchange. Seeding density defines cell behaviour in this model. With low densities cells attach to the membranes and flatten. Increasing density leads to spontaneous formation of aggregates which are immobilized between the capillaries.
为了将肝细胞固定在生物反应器中，开发了一种具有独立灌注的三维编织毛细血管的多隔室毛细血管膜培养模型。这使得细胞能够进行空间重组，并通过更有效的氧合作用和代谢物交换提高传质性能。接种密度决定了该模型中的细胞行为。低密度时，细胞附着在膜上并变平。密度增加会导致聚集体的自发形成，这些聚集体固定在毛细血管之间。
