Naruse K, Sakai Y, Nagashima I, Jiang G X, Suzuki M, Muto T
First Department of Surgery, Faculty of Medicine, University of Tokyo, Japan.
Int J Artif Organs. 1996 Oct;19(10):605-9.
We previously developed a new bioreactor of the bioartificial liver composed of non-woven fabric. We have also experimented with hepatocyte spheroids, with the aim of improving the efficiency of this NWF bioreactor. In this study, we compared the efficiencies of NWF bioreactors employing hepatocyte spheroids versus single hepatocytes. Hepatocytes were isolated from a whole pig liver by Seglen's method. 1.0 x 10(10) single hepatocytes were immobilized in the NWF bioreactor. Another 1.0 x 10(10) hepatocytes were allowed to form spheroids by 24 hr suspension culture in a 4-L culture vessel, before being immobilized in the bioreactor. Hepatocyte spheroids were found to be functionally superior, on a per-cell basis, to single hepatocytes in the NWF bioreactor. However, the NWF bioreactor employing hepatocyte spheroids exhibited lower efficiency than thaT employing single hepatocytes, because the total number of the hepatocytes had decreased during the 24 hr suspension culture.
我们之前开发了一种由无纺布组成的生物人工肝新型生物反应器。我们还对肝细胞球体进行了实验，目的是提高这种无纺布生物反应器的效率。在本研究中，我们比较了使用肝细胞球体的无纺布生物反应器与使用单个肝细胞的无纺布生物反应器的效率。肝细胞通过Seglen法从整个猪肝中分离出来。将1.0×10¹⁰个单个肝细胞固定在无纺布生物反应器中。另外1.0×10¹⁰个肝细胞在4升培养容器中通过24小时悬浮培养形成球体，然后再固定在生物反应器中。发现在无纺布生物反应器中，基于每个细胞而言，肝细胞球体在功能上优于单个肝细胞。然而，使用肝细胞球体的无纺布生物反应器表现出的效率低于使用单个肝细胞的无纺布生物反应器，因为在24小时悬浮培养期间肝细胞总数减少了。
