Bain G, Gottlieb D I
Washington University School of Medicine, Department of Anatomy and Neurobiology, St. Louis, MO 63110.
Biochem Biophys Res Commun. 1994 May 16;200(3):1252-6. doi: 10.1006/bbrc.1994.1585.
The pluripotent mouse embryonal carcinoma cell line P19 provides an excellent model system to study the mechanisms by which retinoic acid (RA) exerts its biological effects. When aggregated and exposed to low concentrations of RA, P19 cells differentiate into neuron- and glial-like cells. The diverse biological effects of RA are mediated by two families of receptors localized in the cell nucleus, the retinoic acid receptors (RARs) and the retinoid X receptors (RXRs). Each family consists of three members designated alpha, beta and gamma. While the patterns of expression of the RARs have been studied in P19 cells, similar data for the RXRs have not been available. We demonstrate here that these receptors are expressed in P19 and are regulated during RA-induced differentiation. We also show that pluripotent mouse embryonic stem cells express the RXRs as well.
多能性小鼠胚胎癌细胞系P19为研究视黄酸（RA）发挥其生物学效应的机制提供了一个极佳的模型系统。当聚集并暴露于低浓度的RA时，P19细胞会分化为神经元样细胞和神经胶质样细胞。RA的多种生物学效应由位于细胞核中的两类受体介导，即视黄酸受体（RARs）和类视黄醇X受体（RXRs）。每个家族由三个成员组成，分别命名为α、β和γ。虽然已经在P19细胞中研究了RARs的表达模式，但关于RXRs的类似数据尚未可得。我们在此证明这些受体在P19中表达，并在RA诱导的分化过程中受到调控。我们还表明多能性小鼠胚胎干细胞也表达RXRs。
