Jonk L J, de Jonge M E, Vervaart J M, Wissink S, Kruijer W
Hubrecht Laboratorium, Netherlands Institute for Developmental Biology, Utrecht.
Dev Biol. 1994 Feb;161(2):604-14. doi: 10.1006/dbio.1994.1056.
The vitamin A derivative retinoic acid (RA) is involved in vertebrate anteroposterior axis formation and cellular differentiation and has been shown to modulate the expression of a number of genes implicated in the control of early embryonal development. Under defined culture conditions, all-trans-RA induces differentiation of P19 embryonal carcinoma cells into neural derivatives. In this report, we describe the isolation and partial characterization of 14 RA-regulated genes from P19 cells committed to differentiate along the neural pathway. In addition to the previously recognized genes encoding vimentin, heat stable antigen, neural cadherin, Hox-B2, F52/MacMARCKS, thymosin beta 4b, and the murine homolog of COUP-TF1, we identified 7 novel genes, 5 of which are predominantly expressed in developing neural tissues as shown by in situ hybridization. The results confirm the usefulness of the P19 system in the isolation and study of the regulation of developmentally expressed genes.
维生素A衍生物视黄酸（RA）参与脊椎动物前后轴的形成和细胞分化，并且已显示其可调节许多与早期胚胎发育控制相关的基因的表达。在特定的培养条件下，全反式视黄酸可诱导P19胚胎癌细胞分化为神经衍生物。在本报告中，我们描述了从致力于沿神经途径分化的P19细胞中分离出14个视黄酸调节基因并对其进行部分特性分析。除了先前已识别的编码波形蛋白、热稳定抗原、神经钙黏蛋白、Hox-B2、F52/MacMARCKS、胸腺素β4b以及COUP-TF1的小鼠同源物的基因外，我们还鉴定出7个新基因，其中5个通过原位杂交显示在发育中的神经组织中主要表达。结果证实了P19系统在分离和研究发育表达基因调控方面的有用性。
