Kwon-Chung K J, Chang Y C
Laboratory of Clinical Investigation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892.
Int J Syst Bacteriol. 1994 Apr;44(2):209-13. doi: 10.1099/00207713-44-2-209.
We cloned the 5S rRNA gene and determined its organization in the four genes encoding rRNAs in a ribosomal DNA repeat unit of Filobasidiella neoformans, the teleomorph of Cryptococcus neoformans. The 5S rRNA gene contained 118 nucleotides and was located 1 kb upstream from the 18S rRNA gene within the 8.6-kb fragment of the ribosomal DNA repeat unit. The sequence of the 5S rRNA gene from F. neoformans was more similar to the sequence of the 5S rRNA gene from Tremella mesenterica than to the sequences of the 5S rRNA genes from Filobasidium species. The arrangement of the rRNA genes in F. neoformans closely resembles the arrangement of the rRNA genes in mushrooms such as Schizophyllum commune, Agaricus bisporus, and Coprinus cinereus in that the 5S rRNA-coding region not only is located within the repeat unit that encodes the other rRNAs but also is transcribed in the same direction as the other rRNA genes. This is the first description of the arrangement of rRNA genes in a species belonging to the Heterobasidiomycetes.
我们克隆了新型隐球菌（丝孢酵母属，新型隐球菌的有性型）核糖体DNA重复单元中编码rRNA的四个基因中的5S rRNA基因，并确定了其结构。5S rRNA基因包含118个核苷酸，位于核糖体DNA重复单元8.6 kb片段内18S rRNA基因上游1 kb处。新型隐球菌5S rRNA基因的序列与银耳5S rRNA基因的序列比与丝孢菌属5S rRNA基因的序列更相似。新型隐球菌中rRNA基因的排列与裂褶菌、双孢蘑菇和灰盖鬼伞等蘑菇中rRNA基因的排列非常相似，因为5S rRNA编码区不仅位于编码其他rRNA的重复单元内，而且转录方向与其他rRNA基因相同。这是对异担子菌纲一个物种中rRNA基因排列的首次描述。
