Llorente L, Richaud-Patin Y, Wijdenes J, Alcocer-Varela J, Maillot M C, Durand-Gasselin I, Fourrier B M, Galanaud P, Emilie D
Department of Immunology and Rheumatology, Instituto Nacional de la Nutricion Salvador Zubirán, Mexico D.F., México.
Eur Cytokine Netw. 1993 Nov-Dec;4(6):421-7.
Interleukin-10 (IL-10) production by B lymphocytes has previously been demonstrated for malignant cells and for in vitro activated normal B cells. Spontaneous in vivo production of IL-10 by normal B lymphocytes has only been demonstrated in mice, in which autoreactive Ly 1 + B cells are involved. In the present study, spontaneous expression of the IL-10 gene by peripheral blood mononuclear cells was investigated in systemic lupus erythematosus (SLE), a human disease involving autoreactive B cells. Of the 47 SLE patients tested by coupled reverse transcriptase-polymerase chain reaction, 34 scored positive, contrasting with only 1 positive out of 34 normal subjects (p < 0.001). Spontaneous in vitro production of IL-10 by PBMC, determined using an ELISA assay, was 33 times higher in SLE than in controls (2623 +/- 728 pg/ml vs 79.3 +/- 34.5 pg/ml, respectively) (p < 0.001). The level of production of IL-10 in SLE was unrelated to either clinical or biological markers of disease activity. Among PBMC, monocytes and B lymphocytes both contributed to IL-10 production, whereas T cells did not. IL-10 overproduction in SLE suggests that this Th2-type interleukin plays a role in the production of autoantibodies through pathways involving both paracrine production by monocytes and autocrine IL-10 production by autoreactive B cells.
此前已证实，B淋巴细胞可产生白细胞介素10（IL-10），这在恶性细胞以及体外活化的正常B细胞中均有体现。正常B淋巴细胞在体内自发产生IL-10的现象仅在小鼠中得到证实，其中涉及自身反应性Ly 1 + B细胞。在本研究中，我们对系统性红斑狼疮（SLE）患者外周血单个核细胞中IL-10基因的自发表达进行了研究，SLE是一种涉及自身反应性B细胞的人类疾病。在通过逆转录聚合酶链反应检测的47例SLE患者中，34例呈阳性，相比之下，34名正常受试者中仅有1例呈阳性（p < 0.001）。使用酶联免疫吸附测定法测定，SLE患者外周血单个核细胞（PBMC）体外自发产生的IL-10比对照组高33倍（分别为2623 +/- 728 pg/ml和79.3 +/- 34.5 pg/ml）（p < 0.001）。SLE中IL-10的产生水平与疾病活动的临床或生物学标志物均无关。在外周血单个核细胞中，单核细胞和B淋巴细胞均对IL-10的产生有贡献，而T细胞则没有。SLE中IL-10的过量产生表明，这种Th2型白细胞介素通过涉及单核细胞旁分泌产生和自身反应性B细胞自分泌IL-10产生的途径，在自身抗体的产生中发挥作用。
