Yamaura M, Nakamura T, Kanou A, Miura T, Ohara H, Suzuki K
Department of Orthopedic Surgery, School of Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan.
Bone Miner. 1994 Jan;24(1):33-42. doi: 10.1016/s0169-6009(08)80129-8.
We performed the dosing experiment to establish whether estrogen administration has any beneficial effects on the mass and the turnover of bone in ovariectomized rats taking a mild dose of thyroxin. Thirty-five Wistar rats, 28 weeks of age, received ovariectomies (OVX) or sham operations and were divided into five groups. Group 1 was the sham group, Groups 2-5 were ovariectomized. Group 2 was the OVX-control, Group 3 treated with thyroxin 30 micrograms/kg/day (T4), Group 4, 17 beta-estradiol 0.3 mg/kg/week (E2), and Group 5, the combination of T4 and E2. The duration of the experiment was 12 weeks. At the end of the experiment, serum chemistries were measured. Bone minerals in the femur were determined with single photon absorptiometry and bone turnover was assessed histomorphometrically. Alkaline-phosphatase increased in Group 3 (OVX-T4), but it reduced in Groups 4 (OVX-E2) and 5 (OVX-T4 + E2). Bone minerals decreased in Groups 2 (OVX) and 3. In Group 4, it was preserved at the same level as in Group 1. Group 5 showed a significant increase of bone mass compared with Group 1. Eroded surface and osteoid surface increased in Groups 2 and 3 and they were reduced in Groups 4 and 5. Bone volume and mineral apposition rate were at a maximum in Group 5. This study demonstrated that 17 beta-estradiol was capable of preventing the bone mass decrease by regulating the turnover in ovariectomized rats taking a mild dose of thyroxin. Osteoblast function appeared to be stimulated in combination with 17 beta-estradiol and thyroxin.
我们进行了给药实验，以确定在服用小剂量甲状腺素的去卵巢大鼠中，给予雌激素是否对骨量和骨转换有任何有益影响。35只28周龄的Wistar大鼠接受了卵巢切除术（OVX）或假手术，并被分为五组。第1组为假手术组，第2 - 5组为去卵巢组。第2组为OVX对照组，第3组每天用30微克/千克甲状腺素（T4）治疗，第4组每周用0.3毫克/千克17β - 雌二醇（E2）治疗，第5组为T4与E2联合治疗。实验持续时间为12周。实验结束时，检测了血清化学指标。用单光子吸收法测定股骨中的骨矿物质，并通过组织形态计量学评估骨转换。碱性磷酸酶在第3组（OVX - T4）中升高，但在第4组（OVX - E2）和第5组（OVX - T4 + E2）中降低。第2组（OVX）和第3组的骨矿物质减少。在第4组中，其保持在与第1组相同的水平。与第1组相比，第5组的骨量显著增加。侵蚀表面和类骨质表面在第2组和第3组中增加，而在第4组和第5组中减少。骨体积和矿物质沉积率在第5组中最高。本研究表明，17β - 雌二醇能够通过调节服用小剂量甲状腺素的去卵巢大鼠的骨转换来预防骨量减少。17β - 雌二醇与甲状腺素联合似乎能刺激成骨细胞功能。
