Aihara N, Mizukawa K, Koide K, Mabe H, Nishino H
Department of Neurosurgery, Nagoya City University Medical School, Japan.
Brain Res Bull. 1994;33(5):483-8. doi: 10.1016/0361-9230(94)90072-8.
We grafted fetal striatal cells in ischemic rat models, and investigated graft survival/growth, GABA release, GABAA receptor reorganization and functional recovery. One hour intraluminal occlusion of the middle cerebral artery (MCA) induced ischemic infarct in the lateral part of the striatum and adjacent cortex. In ischemic rats, the acquisition of Morris' water-maze learning was significantly slower than that of control rats. In these animals GABA level in the globus pallidus, detected by microdialysis, was about the half of that of controls. However, after the grafts of fetal striatal cells in the striatopallidum, the acquisition was improved, thus no difference was observed in the time course of learning curves in control and grafted animals. GABA level recovered to almost normal level by the graft. It further increased by the treatment of a GABA uptake blocker (nipecotic acids) in the perfusion. In the grafts, GABAA receptor organization detected by autoradiography using [3H] labeled SR95531 was restored for more than 1 year after the graft. Data suggest that fetal striatal cell grafts in infarct striatum may partially reconstruct striatopallidal GABA projection and reorganize GABAA receptor. This might be a basis of improvement of function.
我们将胎儿纹状体细胞移植到缺血大鼠模型中，研究移植物的存活/生长、γ-氨基丁酸（GABA）释放、GABAA受体重组及功能恢复情况。大脑中动脉（MCA）管腔内闭塞1小时可在纹状体外侧部和邻近皮质诱发缺血性梗死。在缺血大鼠中，Morris水迷宫学习的获得明显慢于对照大鼠。通过微透析检测发现，这些动物苍白球中的GABA水平约为对照动物的一半。然而，将胎儿纹状体细胞移植到纹状体苍白球后，学习能力得到改善，因此在对照动物和移植动物的学习曲线时间进程中未观察到差异。移植物使GABA水平恢复到几乎正常水平。在灌注中使用GABA摄取阻滞剂（哌啶酸）治疗后，GABA水平进一步升高。在移植物中，使用[3H]标记的SR95531通过放射自显影检测到的GABAA受体组织在移植后1年多的时间里得以恢复。数据表明，梗死纹状体中的胎儿纹状体细胞移植可能部分重建纹状体苍白球GABA投射并重组GABAA受体。这可能是功能改善的基础。
