Arai R, Jacobowitz D M, Deura S
Department of Anatomy, Fujita Health University School of Medicine, Aichi, Japan.
Brain Res Bull. 1994;33(5):595-614. doi: 10.1016/0361-9230(94)90086-8.
The localization of three calcium-binding proteins, calretinin, calbindin-D28k, and parvalbumin, in the rat thalamus was immunohistochemically examined. a) Some thalamic regions revealed cells almost exclusively containing one of the calcium-binding proteins. For example, almost only calretinin-stained cells were found in the central medial and paraventricular nuclei. Calbindin-D28k-stained cells were mostly found in the centrolateral, interanteromedial, anteromedial, and posterior nuclei. Only parvalbumin-positive cells were found in the central part of the reticular nucleus. b) Other regions expressed overlap between the distributions of two cell components composed of different calcium-binding proteins. For example, both calretinin-stained cells and calbindin-D28k-labeled cells were found in the lateroposterior, intermediodorsal, rhomboid, and reuniens nuclei. c) Other regions showed no cells stained for any of the calcium-binding proteins. For example, generally no calcium-binding protein was detected in neurons of the anterodorsal, anteroventral, ventrolateral, ventral posterolateral, ventral posteromedial, or gelatinosus nuclei, or of the central part of the mediodorsal nucleus. These three proteins serve as useful marker for localizing subpopulations of neurons within the thalamus.
采用免疫组织化学方法研究了三种钙结合蛋白（钙视网膜蛋白、钙结合蛋白-D28k和小白蛋白）在大鼠丘脑的定位。a）一些丘脑区域显示细胞几乎只含有一种钙结合蛋白。例如，在中央内侧核和室旁核中几乎只发现了钙视网膜蛋白染色的细胞。钙结合蛋白-D28k染色的细胞主要见于中央外侧核、前内侧间核、前内侧核和后核。在网状核中央部分仅发现了小白蛋白阳性细胞。b）其他区域在由不同钙结合蛋白组成的两种细胞成分分布之间存在重叠。例如，在丘脑后外侧核、背内侧核、菱形核和连合核中均发现了钙视网膜蛋白染色的细胞和钙结合蛋白-D28k标记的细胞。c）其他区域未显示任何钙结合蛋白染色的细胞。例如，在前背核、前腹核、腹外侧核、腹后外侧核、腹后内侧核或胶状核，或背内侧核中央部分的神经元中，通常未检测到钙结合蛋白。这三种蛋白质可作为定位丘脑内神经元亚群的有用标记物。
