Ghosh P, Sica A, Young H A, Ye J, Franco J L, Wiltrout R H, Longo D L, Rice N R, Komschlies K L
Laboratory of Experimental Immunology, NCI-FCRDC, Frederick, Maryland 21702-1201.
Cancer Res. 1994 Jun 1;54(11):2969-72.
It has recently been shown that T-cell signal transduction molecules are altered in tumor-bearing mice. We have examined the expression of NF kappa B/Rel family proteins in T-cells from mice bearing Renca, a murine renal carcinoma. T-cells from Renca-bearing mice expressed undetectable levels of nuclear c-Rel, NF kappa B p65, and p50; however, two shorter forms of p50 (p48 and p46), truncated at the NH2-terminus, were present exclusively in the nucleus and were able to bind DNA. These T-cells have reduced expression of gamma-interferon mRNA. In mice successfully treated with flavone 8-acetic acid and recombinant human interleukin 2, the T-cells expressed normal levels of all three nuclear NF kappa B/Rel proteins. These results suggest that alterations in transcription factors may accompany changes in signal transduction molecules in T-cells from tumor-bearing animals; however, the changes are reversed with successful biological therapy.
最近研究表明，荷瘤小鼠的T细胞信号转导分子发生了改变。我们检测了携带Renca（一种小鼠肾癌）的小鼠T细胞中NF-κB/Rel家族蛋白的表达。来自荷Renca小鼠的T细胞中，核c-Rel、NF-κB p65和p50的表达水平检测不到；然而，两种在NH2末端截短的较短形式的p50（p48和p46）仅存在于细胞核中，并且能够结合DNA。这些T细胞中γ-干扰素mRNA的表达降低。在用黄酮8-乙酸和重组人白细胞介素2成功治疗的小鼠中，T细胞表达所有三种核NF-κB/Rel蛋白的正常水平。这些结果表明，转录因子的改变可能伴随荷瘤动物T细胞信号转导分子的变化；然而，成功的生物治疗可使这些变化逆转。
