39,X小鼠和40,XX小鼠中Rps4 mRNA的表达水平。

Expression level of Rps4 mRNA in 39,X mice and 40,XX mice.

作者信息

Omoe K, Endo A

机构信息

Department of Hygiene and Preventive Medicine, Yamagata University School of Medicine, Japan.

出版信息

Cytogenet Cell Genet. 1994;67(1):52-4. doi: 10.1159/000133796.

DOI:10.1159/000133796

PMID:8187552

Abstract

Contrary to the effects of X-chromosome monosomy in humans (Turner syndrome), XO mice are fertile and anatomically normal. The human RPS4X gene encodes ribosomal protein S4 and escapes X-chromosome inactivation, and its haplo-insufficiency has been suspected to contribute to Turner syndrome. Therefore, we compared the expression level of mRNA of Rps4 (the mouse homolog of RPS4X) between XX and XO mice using Northern blot analysis. The XO/XX ratio of Rps4 mRNA obtained from Northern blot analysis was 0.9. There was no difference in the expression level of Rps4 mRNA between XX and XO mice. The difference in the RPS4/Rps4 transcription pattern between humans and mice may contribute, at least in part, to the phenotypic difference in monosomy X between humans and mice.

摘要

与人类X染色体单体性的影响（特纳综合征）相反，XO小鼠具有生育能力且解剖结构正常。人类RPS4X基因编码核糖体蛋白S4且逃避X染色体失活，其单倍体不足被怀疑与特纳综合征有关。因此，我们使用Northern印迹分析比较了XX和XO小鼠之间Rps4（RPS4X的小鼠同源物）mRNA的表达水平。从Northern印迹分析获得的Rps4 mRNA的XO/XX比值为0.9。XX和XO小鼠之间Rps4 mRNA的表达水平没有差异。人类和小鼠之间RPS4/Rps4转录模式的差异可能至少部分导致了人类和小鼠之间X单体性的表型差异。