Maguire B, Whitaker D, Carrello S, Spagnolo D
Hospital Service, Queen Elizabeth II Medical Centre, Nedlands, Western Australia.
Diagn Cytopathol. 1994;10(2):130-4. doi: 10.1002/dc.2840100207.
Formol sublimate-fixed cell blocks derived from 129 malignant pleural (and some peritoneal) effusions, 8 benign effusions with reactive mesothelial cells, and 23 FNA specimens, were immunostained with monoclonal antibody Ber-EP4 to assess its ability to distinguish malignant mesothelioma (MM) from carcinoma. Only 2 of 44 (4%) well-characterized MM were Ber-EP4+, while none of 8 benign mesothelial proliferations reacted with the antibody. Fifty-seven percent of 23 pulmonary adenocarcinomas (AC) and 60% of 43 pulmonary carcinomas of all other histological types were Ber-EP4+. Of 40 metastatic AC originating from breast, gastrointestinal tract, ovary, endometrium, and kidney, 80% were Ber-EP4+. The predictive value of positive Ber-EP4 staining in distinguishing AC from MM was 96%. The predictive value of a negative Ber-EP4 in excluding MM was 70%, when the differential diagnosis was adenocarcinoma. These results suggest that Ber-EP4 is helpful in differentiating MM and AC if used together with other discriminating antibodies.
取自129例恶性胸腔（以及部分腹腔）积液、8例伴有反应性间皮细胞的良性积液和23例细针穿刺活检标本的甲醛升汞固定细胞块，用单克隆抗体Ber-EP4进行免疫染色，以评估其区分恶性间皮瘤（MM）和癌的能力。在44例特征明确的MM中，只有2例（4%）为Ber-EP4阳性，而8例良性间皮增生均未与该抗体发生反应。23例肺腺癌（AC）中有57%以及43例其他所有组织学类型的肺癌中有60%为Ber-EP4阳性。源自乳腺、胃肠道、卵巢、子宫内膜和肾脏的40例转移性AC中，80%为Ber-EP4阳性。Ber-EP4染色阳性在区分AC与MM方面的预测价值为96%。当鉴别诊断为腺癌时，Ber-EP4阴性在排除MM方面的预测价值为70%。这些结果表明，如果与其他鉴别抗体一起使用，Ber-EP4有助于区分MM和AC。
