替换的v-src和env基因在劳氏肉瘤病毒鸭适应性变体中的作用。

Role of replaced v-src and env genes in the duck-adapted variant of Rous sarcoma virus.

作者信息

Hlozánek I, Rynditch A V, Mikhailik A

机构信息

Institute of Molecular Genetics, Academy of Sciences of the Czech Republic, Praha.

出版信息

Folia Biol (Praha). 1993;39(4):203-10.

PMID:8187899

Abstract

The env and v-src genes of a duck-adapted variant of Rous sarcoma virus were replaced for corresponding genes from parental chick-derived virus. The generation of viral constructs with replaced genes is described. DNAs of viruses with replaced genes were assayed on chick and duck embryo fibroblasts by transfection assays. Transformation efficiency was measured by the focus assay and multiplication of virus by the reverse transcriptase assay. Duck-adapted virus with replaced env gene lost the higher transformation efficiency for duck cells, whereas replacement of the v-src gene had no effect on its host-specific transformation activity.

摘要

劳氏肉瘤病毒鸭适应变种的env和v-src基因被来自亲本鸡源病毒的相应基因所取代。文中描述了具有替换基因的病毒构建体的产生过程。通过转染试验在鸡胚成纤维细胞和鸭胚成纤维细胞上对具有替换基因的病毒DNA进行了检测。通过焦点试验测定转化效率，通过逆转录酶试验测定病毒增殖情况。具有替换env基因的鸭适应病毒对鸭细胞失去了较高的转化效率，而v-src基因的替换对其宿主特异性转化活性没有影响。