Cole M F, Evans M, Fitzsimmons S, Johnson J, Pearce C, Sheridan M J, Wientzen R, Bowden G
Department of Microbiology, Georgetown University Medical Center, Washington, D.C. 20007.
Infect Immun. 1994 Jun;62(6):2165-8. doi: 10.1128/iai.62.6.2165-2168.1994.
As part of a longitudinal study of the relationship between bacterial colonization and the secretory immune response, 367 isolates of pioneer viridans streptococci collected from 40 breast- and bottle-fed neonates within the first month postpartum were tested for the production of immunoglobulin A1 (IgA1) protease and glycosidases. Fifty percent of the streptococci isolated produced IgA1 protease, including all isolates of Streptococcus oralis and S. sanguis, 60.7% of S. mitis biovar 1 isolates, and some isolates that could not be identified. Three cleavage patterns of alpha 1 heavy chains were observed. Six isolates of S. mitis biovar 1 that did not produce IgA1 protease attacked the alpha 1 chain. Incubation of IgA1 protease-negative S. mitis biovar 1 isolates with IgA1, either prior to or together with S. sanguis, rendered the IgA1 paraprotein resistant to cleavage by the IgA1 protease of S. sanguis. The ability of some pioneer streptococci in the human oral cavity to produce IgA1 protease and of others to modify the susceptibility of IgA1 to cleavage by IgA1 protease perhaps enhances their ability to survive in this habitat.
作为一项关于细菌定植与分泌性免疫反应之间关系的纵向研究的一部分，对产后第一个月内从40名母乳喂养和奶瓶喂养的新生儿中收集的367株早期草绿色链球菌分离株进行了免疫球蛋白A1（IgA1）蛋白酶和糖苷酶产生情况的检测。分离出的链球菌中有50%产生IgA1蛋白酶，包括所有口腔链球菌和血链球菌分离株、60.7%的缓症链球菌1型生物变种分离株以及一些无法鉴定的分离株。观察到α1重链的三种裂解模式。六株不产生IgA1蛋白酶的缓症链球菌1型生物变种分离株可裂解α1链。在与血链球菌共同培养之前或同时，将不产生IgA1蛋白酶的缓症链球菌1型生物变种分离株与IgA1一起孵育，可使IgA1副蛋白对血链球菌的IgA1蛋白酶裂解产生抗性。人类口腔中的一些早期链球菌产生IgA1蛋白酶的能力以及其他链球菌改变IgA1对IgA1蛋白酶裂解敏感性的能力，可能增强了它们在该生境中的生存能力。
