Hyatt D R, ter Huurne A A, van der Zeijst B A, Joens L A
Department of Veterinary Science, University of Arizona, Tucson 85721.
Infect Immun. 1994 Jun;62(6):2244-8. doi: 10.1128/iai.62.6.2244-2248.1994.
The role of the Serpulina hyodysenteriae hemolysin encoded by the tlyA gene in the pathogenesis of swine dysentery (SD) was studied. tlyA mutants of two S. hyodysenteriae strains (B204 and C5) were tested for virulence in pigs. None of the animals developed SD. However, after infection with wild-type strain B204 or C5, the incidence of SD was 100 or 60%, respectively. Thus, the tlyA-encoded hemolysin of S. hyodysenteriae is an important virulence factor in SD. The potential of these mutants to protect pigs against challenge with a virulent S. hyodysenteriae strain was also studied. After challenge with wild-type strain B204, 50% of pigs previously inoculated with the B204 tlyA mutant were protected, whereas all control pigs contracted SD. None of the pigs previously inoculated with the C5 tlyA mutant developed SD upon challenge with wild-type strain B204, whereas 40% of the control pigs developed SD in this experiment. Thus, previous colonization with S. hyodysenteriae tlyA mutants in pigs provides partial protection against challenge with a virulent S. hyodysenteriae strain.
研究了由tlyA基因编码的猪痢疾短螺旋体溶血素在猪痢疾（SD）发病机制中的作用。对两株猪痢疾短螺旋体（B204和C5）的tlyA突变体进行了猪的毒力测试。没有动物发生猪痢疾。然而，在用野生型菌株B204或C5感染后，猪痢疾的发病率分别为100%或60%。因此，猪痢疾短螺旋体的tlyA编码溶血素是猪痢疾中的一个重要毒力因子。还研究了这些突变体保护猪免受强毒猪痢疾短螺旋体菌株攻击的潜力。在用野生型菌株B204攻击后，50%先前接种B204 tlyA突变体的猪受到保护，而所有对照猪都感染了猪痢疾。在用野生型菌株B204攻击时，先前接种C5 tlyA突变体的猪均未发生猪痢疾，而在该实验中40%的对照猪发生了猪痢疾。因此，猪先前被猪痢疾短螺旋体tlyA突变体定殖可提供部分保护，使其免受强毒猪痢疾短螺旋体菌株的攻击。
