从鸡胚胎骨骼肌中分离出的一个编码新型RSP基序的部分连接cDNA。
A partial connectin cDNA encoding a novel type of RSP motifs isolated from chicken embryonic skeletal muscle.
作者信息
Maruyama K, Endo T, Kume H, Kawamura Y, Kanzawa N, Kimura S, Kawashima S, Maruyama K
机构信息
Department of Molecular Biology, Tokyo Metropolitan Institute of Medical Science.
出版信息
J Biochem. 1994 Jan;115(1):147-9. doi: 10.1093/oxfordjournals.jbchem.a124290.
A partial cDNA encoding 811 amino acids of connectin (titin), a giant elastic protein of muscle (3,000 kDa), was cloned from a chicken embryonic skeletal muscle cDNA library using antibodies to muscle connectin. The encoded product was the C terminal segment of connectin. The predicted sequences consisted of 5 type II motifs (immunoglobulin C2 type) separated by 5 interdomain insertions. One interdomain insertion had significant homology (RSP) to KSP repeats found in human cardiac C-terminal connectin and another had a high sequence homology to porin (67.7%; 31 amino acids).
利用针对肌肉连接蛋白的抗体，从鸡胚胎骨骼肌cDNA文库中克隆出了一个编码连接蛋白（肌联蛋白）811个氨基酸的部分cDNA，连接蛋白是一种巨大的肌肉弹性蛋白（3000 kDa）。编码产物为连接蛋白的C末端片段。预测序列由5个II型基序（免疫球蛋白C2型）组成，中间由5个结构域间插入序列隔开。其中一个结构域间插入序列与人类心脏C末端连接蛋白中的KSP重复序列具有显著同源性（RSP），另一个与孔蛋白具有高度序列同源性（67.7%；31个氨基酸）。
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