编码猪骨骼肌肌联蛋白的部分cDNA克隆的特性分析：与兔和小鼠骨骼肌肌联蛋白序列的比较
Characterization of a partial cDNA clone encoding porcine skeletal muscle titin: comparison with rabbit and mouse skeletal muscle titin sequences.
作者信息
Fritz J D, Wolff J A, Greaser M L
机构信息
Department of Meat and Animal Science, Waisman Center, University of Wisconsin-Madison 53706.
出版信息
Comp Biochem Physiol B. 1993 Jun;105(2):357-60. doi: 10.1016/0305-0491(93)90241-v.
PMID:8359022
Abstract
- A cDNA fragment encoding 571 amino acid residues of porcine skeletal muscle titin was isolated from total RNA using RNA-PCR. 2. The porcine titin clone hybridized to a large RNA species (> 23 kb) in rabbit cardiac muscle, rabbit skeletal muscle and porcine skeletal muscle. 3. The porcine skeletal muscle titin clone encoded two types of 100-residue motifs and its amino acid sequence was 96% and 93% identical to the corresponding sequence of rabbit and mouse skeletal muscle titin, respectively. These results suggest that the titin sequence is highly conserved among mammalian species.
摘要
- 使用RNA-PCR从总RNA中分离出编码猪骨骼肌肌联蛋白571个氨基酸残基的cDNA片段。2. 猪肌联蛋白克隆与兔心肌、兔骨骼肌和猪骨骼肌中的一种大RNA种类（> 23 kb）杂交。3. 猪骨骼肌肌联蛋白克隆编码两种类型的100个残基的基序，其氨基酸序列与兔和小鼠骨骼肌肌联蛋白的相应序列分别有96%和93%的同一性。这些结果表明肌联蛋白序列在哺乳动物物种中高度保守。
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