Stepniakowski K, Budzikowski A, Loń S, Szczepańska-Sadowska E
Department of Hypertension, Medical Academy of Warsaw, Poland.
J Auton Nerv Syst. 1994 Apr;47(1-2):33-43. doi: 10.1016/0165-1838(94)90063-9.
The purpose of the present study was to compare influence of central arginine vasopressin (AVP) and of atrial natriuretic peptide (ANP) on control of arterial blood pressure (MAP) and heart rate (HR) in normotensive (WKY) and spontaneously hypertensive (SHR) rats. Three series of experiments were performed on 30 WKY and 30 SHR, chronically instrumented with guide tubes in the lateral ventricle (LV) and arterial and venous catheters. MAP and HR were monitored before and after i.v. injections of either vehicle or 1, 10 and 50 ng of AVP and 25, 125 and 500 ng of ANP. Sensitivity of cardiac component of baroreflex (CCB), expressed as a slope of the regression line was determined from relationships between systolic arterial pressure (SAP) and HR period (HRp) during phenylephrine (Phe)-induced hypertension and sodium nitroprusside (SN)-induced hypotension. CCB was measured before and after administration of either vehicle, AVP, ANP, or both peptides together. Increases of MAP occurred after LV administration of 1, 10 and 50 ng of AVP in WKY and of 10 and 50 ng in SHR. ANP did not cause significant changes in MAP in both strains as compared to vehicle, but it abolished AVP-induced MAP increase in WKY and SHR. CCB was reduced in WKY and SHR after LV administration of AVP during SN-induced hypotension. In SHR but not in WKY administration of ANP, AVP and ANP + AVP decreased CCB during Phe-induced MAP elevation. The results indicate that centrally applied AVP and ANP exert differential effects on blood pressure and baroreflex control of heart rate in WKY and SHR and suggest interaction of these two peptides in blood pressure regulation at the level of central nervous system.
本研究的目的是比较中枢精氨酸血管加压素（AVP）和心房利钠肽（ANP）对正常血压（WKY）大鼠和自发性高血压（SHR）大鼠动脉血压（MAP）及心率（HR）调控的影响。对30只WKY大鼠和30只SHR大鼠进行了三组实验，这些大鼠长期植入了侧脑室（LV）引导管以及动脉和静脉导管。在静脉注射溶剂或1、10和50 ng的AVP以及25、125和500 ng的ANP之前和之后监测MAP和HR。压力感受性反射的心脏成分（CCB）敏感性以回归直线斜率表示，通过去氧肾上腺素（Phe）诱导高血压和硝普钠（SN）诱导低血压期间收缩期动脉压（SAP）与心率周期（HRp）之间的关系来确定。在给予溶剂、AVP、ANP或两种肽共同给药之前和之后测量CCB。在WKY大鼠中，LV注射1、10和50 ng的AVP后MAP升高，在SHR大鼠中，注射10和50 ng的AVP后MAP升高。与溶剂相比，ANP在两种品系中均未引起MAP的显著变化，但它消除了WKY大鼠和SHR大鼠中AVP诱导的MAP升高。在SN诱导低血压期间，LV注射AVP后，WKY大鼠和SHR大鼠的CCB降低。在SHR大鼠中，而非WKY大鼠中给予ANP、AVP以及ANP + AVP后，在Phe诱导的MAP升高期间CCB降低。结果表明，中枢给予的AVP和ANP对WKY大鼠和SHR大鼠的血压及心率压力感受性反射调控具有不同作用，并提示这两种肽在中枢神经系统水平的血压调节中存在相互作用。
