Wang C Y, Dominguez G, Frey T K
Department of Biology, Georgia State University, Atlanta 30303.
J Virol. 1994 Jun;68(6):3550-7. doi: 10.1128/JVI.68.6.3550-3557.1994.
Plasmids containing a complete cDNA copy of the rubella virus (RUB) genomic RNA were constructed. Transfection into cell culture of genome-length RNA transcribed in vitro from one of these cDNA clones, Robo102, resulted in the production of virus which preserved the genetic and phenotypic characteristics of the parental virus from which the cDNA clone was derived. Prior to construction of the RUB genome-length cDNA clones, the 5'-terminal sequence of the RUB genomic RNA was determined to be 5'CAAUGG...3' following the cap structure. Analysis of the specific infectivity of RUB genomic RNA isolated from virions revealed that in Vero cells, the specific infectivity of RUB genomic RNA is roughly equivalent to that of Sindbis virus genomic RNA. In RUB virion RNA preparations, the subgenomic RNA was detected. It was demonstrated that subgenomic RNA was packaged into RUB virions; however, the presence of the subgenomic RNA was not essential for infectivity of the genomic RNA.
构建了含有风疹病毒（RUB）基因组RNA完整cDNA拷贝的质粒。将其中一个cDNA克隆Robo102体外转录的基因组长度RNA转染到细胞培养物中，产生了保留了该cDNA克隆所源自的亲本病毒的遗传和表型特征的病毒。在构建RUB基因组长度cDNA克隆之前，确定RUB基因组RNA的5'-末端序列在帽结构之后为5'CAAUGG...3'。对从病毒粒子中分离的RUB基因组RNA的比感染性分析表明，在Vero细胞中，RUB基因组RNA的比感染性大致等同于辛德毕斯病毒基因组RNA的比感染性。在RUB病毒粒子RNA制剂中，检测到了亚基因组RNA。已证明亚基因组RNA被包装到RUB病毒粒子中；然而，亚基因组RNA的存在对于基因组RNA的感染性并非必不可少。
